Peptide ligation from alkoxyamine based radical addition.

نویسندگان

  • Thomas Trimaille
  • Laurent Autissier
  • Mamy Daniel Rakotonirina
  • Yohann Guillaneuf
  • Claude Villard
  • Denis Bertin
  • Didier Gigmes
  • Kamel Mabrouk
چکیده

Intermolecular radical 1,2-addition (IRA) of N-tert-butyl-N-(1-diethylphosphono-2,2-dimethylpropyl)aminoxyl (SG1) based alkoxyamines onto activated olefins is used as a tool for peptide ligation. This strategy relies on simple peptide pre-derivatization to obtain (i) a SG1 nitroxide functionalized resin peptide at its N-terminus (SG1-peptide alkoxyamine), (ii) a vinyl functionalized peptide (either at its C-terminus or N-terminus), and does not require any coupling agents.

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عنوان ژورنال:
  • Chemical communications

دوره 50 21  شماره 

صفحات  -

تاریخ انتشار 2014